Reliable ß-Tubulin Loading Control for Mouse Lung Tissue Western Blotting

June 22, 2026

BlueskyReddit
Neurosurgery
University of Florida
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

Beta Tubulin Loading Control Monoclonal Antibody (BT7R)

Catalog Number:

MA5-16308

Clone Number:

N/A

I used this antibody as a loading control for Western blot analysis of mouse lung tissue samples. The antibody performed consistently at a 1:1000 dilution with overnight incubation at 4°C in SuperBlock, producing strong, clean β-tubulin bands with minimal background and excellent reproducibility across samples.

Experimental Design and Results Summary

Applications

Western blot

Sample

Mouse lung tissue lysates

Primary Incubation

1:1000 dilution, overnight at 4°C

Blocking Agent

SuperBlock™ Blocking Buffer

Secondary Incubation

HRP-conjugated anti-mouse IgG, 1:5000 dilution, 1 hour at room temperature

Tertiary Incubation

N/A

Detection

Chemiluminescent detection (ECL)

Results Summary

This antibody provided strong and consistent β-tubulin signals across mouse lung tissue samples and served as a reliable loading control for normalization of protein expression. At a 1:1000 dilution with overnight incubation at 4°C, the antibody produced clear bands with minimal background and required no additional optimization.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Strong signal, low background.

The Bad

No significant issues observed under the tested conditions.

The Bottom Line

Reliable loading control antibody with strong signal and low background, highly recommended for Western blot analysis of tissue lysates.

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