Beckman Coulter
TCR Cβ2 (TRBC2)-APC
D06177
SAM.2
Info: Supplier Page
Similar to kappa and lambda on B cells, TRBC1 and TRBC2 are being used nowadays for evaluating T cell clonality. Although some labs use only TRBC1, we decided to use both because it allows for better resolution of clonal T cells especially when the signal from TRBC1 is dim/equivocal. The choice of fluorochrome (APC) was made because TRBC2 is not as strong an antigen as TRBC1, so we went for a bright dye.
Flow cytometry
Human blood, bone marrow, or tissue
15 minutes at Room Temp
0.5% FBS
N/A
The APC channel (~660nm)
The results were very helpful in resolving normal T cells into either TRBC1 positive or TRBC2 positive. More importantly, it helped us detect clonal proliferations of T cells, including T-CUS (clonal T cells of unknown significance). The image provided just show TRBC2 on its own and how well the separation is.
Good and bright signal.
Quite expensive, and limited utility.
The antibody performed as expected and helped solve a very important problem in T cell immunophenotyping.