Cell Signaling Technology
Histone H3 (K27M Mutant Specific) (D3B5T) Rabbit Monoclonal Antibody
74829
N/A
Info: Supplier Page
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Tested by Western blot on mouse lung tissue at 1:1000 dilution. The antibody produced a strong, clear signal with low background and good reproducibility. I detected both the H3K27M mutant signal and total Histone H3 in my samples. Overall, a reliable antibody for western blot applications.
Western blot
Lung Mouse Tissue
1:1000 overnight 4 C
SuperBlock™ T20 (TBS) Blocking Buffer
1:10000 Rabbit 4 hours RT
Chemiluminescent
Strong and clear western blot signal at 1:1000 dilution using mouse lung tissue. Chemiluminescent detection showed low background and good reproducibility. Both H3K27M mutant and total Histone H3 signals were detected. Overall, the antibody performed reliably for western blot analysis.
Strong signal.
None.
I was happy with the results.