Thermo Fisher Flow Cytometry Antibody: Reliable, Reproducible, Research-Ready

June 10, 2026

BlueskyReddit
Medicine
Weill Cornell Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

ThermoFisher

Product Name:

FOXP3 Monoclonal Antibody (FJK-16s), eFluor 450, eBioscience™

Catalog Number:

48-5773-82

Clone Number:

FJK-16s

This antibody performed exceptionally well in our studies, demonstrating strong specificity, consistent staining, and excellent reproducibility across experiments. The results were robust and reliable, making it a valuable reagent for our research applications.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse intestinal lamina propria lymphocytes

Primary Incubation

Intracellular, 30 minutes at 30°C

Blocking Agent

Anti-CD16/CD32 Fc receptor blocking antibody

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

The antibody demonstrated excellent specificity, sensitivity, and reproducibility in murine immune cell populations. Clear separation of positive and negative populations was observed with minimal background staining. The reagent performed consistently across multiple independent experiments and was suitable for quantitative assessment of immune cell subsets.

DOI or PMID #

38867048

Additional Notes

The antibody was validated in multiple tissues and experimental conditions and showed robust performance with standard flow cytometry staining protocols.

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Summary

The Good

Excellent clear population resolution, and highly reproducible staining across experiments.

The Bad

No major issues encountered. Optimization of antibody concentration may be required for rare cell populations.

The Bottom Line

A reliable, high-quality antibody that provides consistent and reproducible results for flow cytometric analysis. Highly recommended for immunological studies.

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