Reliable NIR Fluorescent NHS Ester for Small-Scale Conjugation with Consistent Signal and Minimal Background from Tocris Bioscience

June 02, 2026

BlueskyReddit
MD Anderson Cancer Center - University of Texas
Cancer System Imaging (Diagnostic Imaging Division)
Lab Technician

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Company:

Tocris Bioscience (Bio-Techne)

Product Name:

NIR Dye s775z, NHS

Catalog Number:

7626

NIR Dye s775z NHS ester (Cat. #7626) from Tocris performed reliably for near-infrared fluorescent labeling applications. The 1 mg format was practical for small-scale conjugation experiments, and the reagent gave consistent signal with minimal background.

Experimental Design and Results Summary

Application

Near-infrared fluorescent labeling / bioconjugation (NHS ester coupling to primary amines on proteins or antibodies)

Starting Material

1 mg lyophilized powder, reconstituted in anhydrous DMSO

Tips

Protect from light at all times; prepare fresh working solutions; use anhydrous conditions during reconstitution to avoid NHS ester hydrolysis

Results Summary

Efficient conjugation to target protein with strong NIR signal and low non-specific background upon imaging

DOI or PMID #

N/A

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Summary

The Good

Strong NIR signal, low background, convenient 1 mg format, and reputable vendor.

The Bad

High MW (1507.22) may affect conjugate behavior; small quantity limits large-scale use.

The Bottom Line

A dependable, high-quality NIR NHS ester dye for small-scale research conjugation when handled carefully under anhydrous, light-protected conditions.

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