Consistent SOD1 staining in mouse hippocampus

SUNY UB
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

Anti-SOD1 Antibody

Catalog Number:

PA5-27240

Clone Number:

N/A

This antibody was used to quantify SOD1 expression associated with oxidative stress responses in astrocytes. Signal quality was consistent across animals and compatible with multi-label confocal imaging workflows.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse brain sections

Primary Incubation

1:1000 overnight at 4°C

Blocking Agent

3% normal goat serum + 0.3% Triton X-100 in PBS

Secondary Incubation

Goat anti-rabbit Alexa Fluor 633, 1:1000, 1 h RT

Tertiary Incubation

NA

Detection

Confocal imaging

Results Summary

The antibody provided robust cytoplasmic staining and enabled reliable quantification of increased SOD1 levels in reactive astrocytes.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Reproducible signal and low nonspecific staining.

The Bad

None significant.

The Bottom Line

Very good antibody for oxidative stress and neuroinflammation studies.

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