Wako
Anti-Iba1 Antibody
019-19741
N/A
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We utilized this antibody to evaluate microglial activation in the mouse hippocampus after astrocyte-specific MOR knockdown. The staining quality was exceptional, allowing for clear visualization of both ramified and activated microglial morphology.
Immunofluorescence
Mouse hippocampal sections
1:1000 overnight at 4°C
3% normal goat serum + 0.3% Triton X-100 in PBS
Goat anti-rabbit Alexa Fluor 633, 1:1000, 1 h RT
NA
Confocal imaging
The antibody provided robust microglial labeling with excellent morphological resolution and minimal background staining, making it well suited for ramification index analysis and co-labeling with mCherry and DAPI.
Excellent morphology detail and reproducible staining.
Requires careful optimization of imaging settings for dense hippocampal regions.
A very dependable antibody for microglia visualization and activation studies.