Cell Signaling Technology
TAZ Monoclonal Antibody
70148S
D3I6D
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We developed a TAZ overexpression system in murine melanoma cells to investigate the effects of TAZ-TEAD signaling in the immune microenvironment. This antibody performed exceptionally well in our system. We have also tested it in human melanoma cell lines, and it works effectively there as well.
Western blot
1014 muring melanoma cells
1:1000, o/n at 4C
5% BSA
1:4000, 1 hour at room temperature
N/A
Chemiluminescence on Biorad ChemiDoc
This helped us validate a doxycycline inducible TAZ overexpression system. We will use it in vivo to study the effects of TAZ expression on the immune microenvironment.
Great signal, and reproducible results.
None.
We've already repurchased, works great in our hands with no major background bands.