An Acceptable Performer for ILT2 Profiling in NK Cells

March 17, 2026

BlueskyReddit
School of Medicine
Lambe Institute for Translational Research
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

ThermoFisher Scientific

Product Name:

CD85j (ILT2) Monoclonal Antibody (HP-F1), Super Bright™ 600

Catalog Number:

63-5129-41

Clone Number:

HP-F1

This antibody provides adequate staining of the inhibitory receptor ILT2 on human peripheral blood NK cells. However, it requires the highest concentration for optimal results, and the resolution may not be ideal.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Human peripheral blood NK cells expanded with feeder cells

Primary Incubation

1:20 dilution, for 20 min at room temperature

Blocking Agent

PBS + 10% FBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Cytek NL2000

Results Summary

This antibody offers sufficient staining of the inhibitory receptor ILT2 on the surface of human peripheral blood NK cells, but requires maximum concentration and the resolution is not optimal.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The resolution is just adequate.

The Bad

The resolution is moderate, making it harder to clearly distinguish low-expressing cells from negatives and requiring careful gating.

The Bottom Line

Expensive and resolution is not good enough, would buy a different conjugate.

Share your experience with other scientists. Write a Review! »

Join the discussion