A Golden Standard Antibody for Precise CD9 Detection on NK Cells

School of Medicine
Lambe Institute for Translational Research
Postdoctoral Researcher

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Quality of Results

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Company:

Miltenyi

Product Name:

CD9 Antibody, Anti-human, PerCP-Vio® 700

Catalog Number:

130-103-992

Clone Number:

SN4 C3-3A2

This antibody provides excellent staining of the CD9 tetraspanin on the surface of human peripheral blood natural killer (NK) cells.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Human peripheral blood NK cells expanded with feeder cells

Primary Incubation

1:10 dilution, for 20 min at room temperature

Blocking Agent

PBS + 10% FBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Cytek NL2000

Results Summary

This antibody offers excellent staining of the CD9 tetraspanin on the surface of human peripheral blood NK cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good resolution.

The Bad

None.

The Bottom Line

This antibody produces a strong signal, but it must be used at the manufacturer-recommended concentration, resulting in a higher cost.

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