High Resolution CD3 Detection in PBMCs with Exceptional Clarity

March 02, 2026

BlueskyReddit
School of Medicine
Lambe Institute for Translational Research
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review
  • Review Image
  • Review Image

Company:

BioLegend

Product Name:

Brilliant Violet 510™ anti-human CD3 Antibody

Catalog Number:

317331

Clone Number:

OKT3

This antibody delivers exceptional staining of the CD3 molecule on the surface of human T cells isolated from PBMCs. Its effectiveness is undeniable, making it an essential tool for precise analysis of immune cells.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

T cells derived from human PBMCs

Primary Incubation

1:20 dilution, for 20 min at room temperature

Blocking Agent

PBS + 10% FBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Cytek NL2000

Results Summary

This antibody offers excellent staining of the CD3 receptor on the surface of human T cells derived from PBMCs.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

  • Review Image
  • Review Image

Summary

The Good

Has good resolution.

The Bad

None.

The Bottom Line

The antibody has great signal, highly recommended.

Share your experience with other scientists. Write a Review! »

Join the discussion