Advanced Immunoblotting Solutions from Proteintech

Cedars Sinai Medical Centre
Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Proteintech

Product Name:

Blotting Accelerator

Catalog Number:

PR20039

I had the opportunity to test the blotting accelerator, and I found it to be an excellent solution for everyday Western blotting. I no longer need to block my membrane. This product worked wonderfully when I used it as a diluent for the antibody, even without blocking the membrane. I incubated the sample for 30 minutes at room temperature, rinsed it with TBST for 1 minute, and then performed the secondary incubation with the same diluent for 15 minutes. This product undoubtedly reduces the workload, allowing me to complete everything in just one day.

Experimental Design and Results Summary

Application

Western Blotting

Starting Material

Antibody

Tips

May need to optimize with difficult proteins.

Results Summary

This product helped me a lot in expediting my work. I was able to finish my western in few hours which is generally a two-day long process. The results were also very clean and nice.

DOI or PMID #

In communication

Related Categories

Image Gallery

Summary

The Good

You can obtain the results faster.

The Bad

The dilutions won't work after one week.

The Bottom Line

It helps speed up the overall process. Highly recommended for abundant proteins.

Join the discussion