CD81 Antibody for EV Marker from Cell Signaling Technology

Pharmacology
UNIVERSITY OF ALABAMA AT BIRMINGHAM
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

CD81 (D5O2Q) Rabbit mAb

Catalog Number:

#10037

Clone Number:

D5O2Q

CD81 antibody was utilized to identify extracellular vesicles (EVs) extracted from the culture media of 3T3 cells. The findings indicated that CD81 is present in the EVs and may serve as a marker for extracellular vesicles.

Experimental Design and Results Summary

Applications

Western blot

Sample

Extracellular vesicles (EVs) from 3T3 cell media

Primary Incubation

1:500 overnight at 4 degrees

Blocking Agent

5% milk in TBS

Secondary Incubation

1 hour at room temperature

Tertiary Incubation

N/A

Detection

ECL

Results Summary

CD81 antibody was used to detect extracellular vesicles (EVs) extracted from 3T3 cell culture media. The results indicated that CD81 is present in the EVs and could serve as a marker for extracellular vesicles.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

It's a good antibody for EV detection.

The Bad

N/A

The Bottom Line

This antibody is good to check EV marker.

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