Quick-Start DNA Quantification Standards by ThermoFisher

May 22, 2025

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Covaris
R&D
Associate Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

ThermoFisher Scientific

Product Name:

Quant-iT™ 1X dsDNA Assay Kits high sensitivity (HS)

Catalog Number:

Q33232

This kit is designed for quantifying dsDNA with an input range of 0.2 to 100 ng, which is optimal for quantifying NGS libraries.

Experimental Design and Results Summary

Application

NGS library prep quantification

Starting Material

Bacterial DNA

Tips

Highly selective to dsDNA over other types of nucleic acids

Results Summary

Used it post-library prep to quantify the output and pool the libraries to run on Illumina platform for NGS.

DOI or PMID #

N/A

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Summary

The Good

Pre-diluted standards and 1x working solution make it really quick.

The Bad

Protocol is written in a confusing manner.

The Bottom Line

This is the standard for DNA quantification kits and having pre-diluted standards make it better than kits like Picogreen.

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