Basal Medium for Differentiation of ES and iPS Cells into Nephron Progenitor Cells

May 22, 2025

BlueskyReddit
UNIVERSITY OF ALABAMA AT BIRMINGHAM
Pharmacology
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

STEMCELL Technologies

Product Name:

TeSR™-E5/E6 Basal Medium

Catalog Number:

#05916

WWe use TeSR™-E6 for induced pluripotent stem (iPS) cell differentiation. This medium is serum- and xeno-free and does not contain any growth factors. Our results indicate that it maintains a stable and effective environment for both embryonic stem (ES) and iPS cell differentiation. Initially, the ES and iPS cells are cultured in Matrigel-coated 6-well plates using TeSR™-E8™ medium. Once the cells reach 50% confluence, we replace the medium with TeSR™-E5/E6 Basal Medium and nephron organoid inducers. The cells are then maintained in this medium thereafter.

Experimental Design and Results Summary

Application

ES and iPS cell differentiation

Starting Material

Matrigel gel coating

Tips

Use it freshly.

Results Summary

We use this TeSR™-E6 for iPS differentiation. It is a serum- and xeno-free medium and does not contain any growth factor. The results showed it maintains a good and stable condition for ES and iPS cell differentiation.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Good basal media for cell differentiation.

The Bad

N/A

The Bottom Line

With sequential inducers in TeSR™-E5/E6 Basal Medium, ES and iPS cells were successfully differentiated into nephron progenitor cell 10 days after and further formed nephron organoid in the 96-well plate.

Join the discussion