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Alexa Fluor® 700 anti-mouse H-2Kb Antibody
116522
AF6-88.5
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The Alexaflour 700 H-2kb antibody is effective in identifying MHC class I-expressing cell populations within immune cells isolated from mouse lungs and bronchoalveolar lavage fluid. It can be utilized for myeloid cells, including dendritic cells, monocytes, macrophages, and neutrophils.
Flow cytometry
Mouse Lungs
Extracellular antibodies at 4C; 30 mins
Fc Shield
BD cytofix/cytoperm
None
5-laser Cytek Aurora
I used this product to identify MHC-1 expressing cells amongst monocytes, dendritic cells, macrophages, and other myeloid cells isolated from the lung / BALF of unimmunized versus intranasally immunized mice. Cells were isolated from BALF using 1 ml of 1% BSA in PBS and from lungs through collagenase digestion; RBC lysis was performed using ACK buffer. Cells were then stained with viability dye followed by extra-cellular antibodies such as CD45, CD90.2, CD19, Siglec-F, CD11b, CD11c, Ly6C, Gr1, MHCII, MHCI.
N/A
Versatile fluorophore for various panels
None so far.
I would recommend the Af700 H-2kb antibody for detection of MHC-I expression in cell populations isolated from the lung, BALF, lymph nodes or spleen.