Efficient Antibody From Abcam With Specific Staining and Little to No Background

February 07, 2025

BlueskyReddit
Wake Forest Baptist Health

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-GLB1/Beta-galactosidase antibody [EPR26120-76]

Catalog Number:

ab305174

Clone Number:

N/A

Nice and specific intracytoplasmic granular staining of hepatocytes in FFPE human adult liver biopsy tissue.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Human Liver

Primary Incubation

Working dilution: 1:500; Duration: overnight; Temperature: 4C

Blocking Agent

BLOXALL and IHC/ISH Super Blocking 110

Secondary Incubation

Conjugate: RRX; Working dilution: 1:250; Duration: 1h; Temperature: 25C

Tertiary Incubation

N/A

Detection

IF imaging

Results Summary

This antibody worked on the first try and gave great specific staining with little background in the liver. This stain will be used to look for senescence in liver biopsy tissue.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Worked in the first attempt itself

The Bad

Nothing so far.

The Bottom Line

Great staining antibody to look for senescence.

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