Gentle, Enzyme-Free Solution From Stemcell Technologies for the Breaking Down of Cells.

University of Louisville
Postdoctoral Researcher

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Company:

Stemcell Technologies

Product Name:

Gentle Cell Dissociation Reagent cGMP

Catalog Number:

100-0485

It is used to isolate intestinal crypts for the establishment of intestinal organoids and to disassemble Matrigel domes when passaging organoid cultures. The action is very specific, and the cells remain viable.

Experimental Design and Results Summary

Application

Cell culture

Starting Material

Intestinal tissue

Tips

Don't overtreat cells with the solutions, as they might die.

Results Summary

Makes it easy to isolate intestinal crypts from tissue with proper incubation and helps in the passaging organoid cultures.

DOI or PMID #

N/A

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Summary

The Good

Works effectively.

The Bad

None.

The Bottom Line

Gentle dissociation reagent which does it job if proper protocol is followed, keeping the cells viable.

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