BCA Kit Designed for Accurate Quantification of Total Protein Content in Various Solutions for Laboratory Applications.

Scripps Research
Department of Immunology and Microbiology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

ThermoFisher

Product Name:

Pierce™ BCA Protein Assay Kit

Catalog Number:

23225

The Pierce BCA Protein Assay includes Reagent A and Reagent B, which need to be pre-mixed. This assay is highly effective for quantifying total protein in various solutions, including cell lysates and purified protein solutions. It is compatible with buffers that contain detergents and provides rapid protein quantification. Additionally, it works reliably for a wide range of proteins expressed in both mammalian and bacterial systems.

Experimental Design and Results Summary

Application

Protein quantification

Starting Material

Protein containing solution

Tips

Make sure to prepare standards in same buffer as protein sample.

Results Summary

This product helped to quantify total protein content in bacterial and cell lysates for normalized loading onto the gel. It also helped quantify total protein in solution post purification of a single protein. The stock solutions could be made and stored at 4C, which made using the kit even easier.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Very easy and quick to use.

The Bad

None so far!

The Bottom Line

I would highly recommend this BCA kit for protein quantification given its versatility to be used across a wide range of proteins and buffers and its quick and easy protocol.

Join the discussion