A Strong cas9 Antibody From Cell Signaling Technology Suitable for Western Blot

Pharmacology
UNIVERSITY OF ALABAMA AT BIRMINGHAM
Research Scientist

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Company:

Cell Signaling Technology

Product Name:

Cas9 (S. pyogenes) (7A9-3A3) Mouse Ab

Catalog Number:

14697S

Clone Number:

7A9-3A3

To check cas9 protein levels after iPS cells were transfected with the px458 vector, which includes the cas9 sequence. The results showed that the cas9 protein is highly expressed.

Experimental Design and Results Summary

Applications

Western blot

Sample

IPS cell

Primary Incubation

1:1000 overnight at 4 degrees

Blocking Agent

5% milk in TBS

Secondary Incubation

1:3000, 1 hour at room temperature

Tertiary Incubation

N/A

Detection

ECL

Results Summary

To check cas9 protein levels after IPS cells were transfected with px458 vector which includes cas9 sequence. The results showed that highly expressed cas9 protein.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Has strong signaling for Western Blot.

The Bad

N/A

The Bottom Line

A good cas9 antibody for Western Blot.

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