Reliable Antibody for FACS From BioLegend

October 17, 2024

BlueskyReddit
AG 61
Leibniz Institute für Virologie
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 700 anti-mouse CD4 Antibody

Catalog Number:

100429

Clone Number:

GK1.5

Reliable antibody for flow cytometry that separates CD4 T cells in mouse samples. The signal is bright, and the populations are clearly defined.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse Cells

Primary Incubation

1/400 in PBS/FCS for 25 minutes at RT

Blocking Agent

CD16/32

Secondary Incubation

None

Tertiary Incubation

None

Detection

FACS

Results Summary

Easy to use FACS antibody that allow to separate CD4 cells in several mouse organs. The staining is clear and reliable and can be used a relatively low concentration.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Bright signal

The Bad

None

The Bottom Line

Definitely recommended for an easy and reliable staining.

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