BosterBio
Mouse CD14 ELISA Kit PicoKine®
EK0695
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CD14 (sCD14), released by macrophages on stimulation with endotoxin, has been used as a marker of gut hyperpermeability. In our study with DSS, we measured the gut permeability to evaluate barrier dysfunction.
ELISA
Serum
Follow the incubation times closely
Absorbance was measured at 450 nm with an ELISA Reader. The CD14 concentration determined the gut permeability in mice treated with DSS.
N/A
Easy to follow the protocol.
Effective method to measure gut permeability if you are unable to do FITC dextran in your experiment.