FITC Anti-Mouse Lineage Cocktail From BioLegend Has a Great Signal

August 09, 2024

BlueskyReddit
Biomedical Sciences
Tulane University School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

FITC anti-mouse lineage cocktail with isotype control

Catalog Number:

133302

Clone Number:

N/A

Bone marrow cells were harvested from the femurs of mice, followed by blocking, and then immunostained with the FITC anti-mouse lineage cocktail for 20 minutes and other immune surface markers. Flow cytometry was carried out and negative selection with this antibody provided the pool of lineage-negative stem cells.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse bone marrow cells

Primary Incubation

20 ul each test, 20 min @ 4 degrees C

Blocking Agent

TruStain FcX™ (anti-mouse CD16/32) Antibody

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

20 ul FITC anti mouse lineage cocktail was used per sample. Bone marrow cells from femurs were immunostained with this antibody. We wanted to characterize the stem cells between the test and control group. this antibody provided good quality signal to separate lineage negative population from the bone marrow cells and helped us perform downstream analysis.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

This antibody works well.

The Bad

Amount required per test is high.

The Bottom Line

Great signal. I highly recommend it.

Share your experience with other scientists. Write a Review! »

Join the discussion