PE Anti-Mouse/Human CD45R/B220 Antibody Ideal for Both Human and Mouse Cells

June 18, 2024

BlueskyReddit
LP2M
University of Cote d'Azur (UCA)
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

PE anti-mouse/human CD45R/B220

Catalog Number:

103207

Clone Number:

RA3-6B2

We are interested in the biology of the lymphocytes B and we need to identify the different subclasses. This antibody, besides the excellent staining, has the advantage of working in both human and mouse samples.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

PBMC

Primary Incubation

20 minutes on ice (0,2ug/100K cells)

Blocking Agent

PBS - 3% BSA

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS

Results Summary

Very easy identification of B lymphocytes subpopulation PE220+. The antibody has strong signal and can be easily couple with others with any cross-reaction.

DOI or PMID #

N/A

Additional Notes

The dilution can be increased, but we never had aspecific staining.

Related Categories

Image Gallery

Summary

The Good

Good for mouse and human samples.

The Bad

N/A

The Bottom Line

Excellent and economic choice to stain both human and murine PBMC.

Share your experience with other scientists. Write a Review! »

Join the discussion