Invitrogen
TRIzol Reagent
15596018
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I use the Trizol reagent to isolate RNA from mammalian cells and tissues. Consistently, I achieved a robust RNA yield suitable for my RT-PCR experiments with this product. Additionally, when preparing RNA for RNAseq experiments, I have found the RNA integrity numbers to be satisfactory.
RNA isolation
Cells or tissues
Saturate tips while pipeting, use in fume hood.
In my experiments, the utilization of Trizol reagent for RNA extraction from mammalian cells consistently yielded abundant RNA suitable for downstream applications. Specifically, RT-PCR experiments demonstrated robust amplification, indicating high-quality RNA extraction. The yield was significantly higher when compared to phenol-chloroform extraction.
N/A
Good reagent with high RNA yield.
Expensive.
Trizol consistently delivers top-notch RNA quality, making it my go-to for extracting RNA from mammalian cells and tissues.