BD Biosciences
BUV395 Rat Anti-Mouse CD19
565965
1D3
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We perform in vivo/in vitro targeting and engineering of mouse B cells. To analyze the on and off-target efficiency, we analyze different immune subsets. After initial gating on CD45+ cells, this CD19 antibody is used to gate on CD19+ B-cell population.
Flow cytometry
Splenocytes from C57BL/6 mouse strain
30 minutes at room temperature
N/A
We used 1:100 dilution of the antibody for 1 million of mouse splenocytes in 100 microliters of the total staining volume. The staining of mouse B cells was obtained and a distinct population of CD19+ cells could be gated.
Specific staining.
Pricing is on higher end.
Good signal, recommended for primary cells.