Decent and Reliable Kit From BD Biosciences for the Measurement of IFN-g

March 15, 2024

UiO
Immunology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BD OptEIA™ Human IFN-γ ELISA Set

Catalog Number:

555142

We are an immunotherapy-focused group interested in CD8 T-cells and their TCRs. We routinely use T-cell-based assays for our experimental purposes, where IFN-g is one of our outputs.

Experimental Design and Results Summary

Application

ELISA

Starting Material

Supernatant

Tips

Include several dilutions of the sample to ensure detection within the standard curve.

Results Summary

The kit performs well in detecting the activated T-cells via ifn-g secretion. This correlates well with CD137 upregulation, with the latter being more sensitive.

DOI or PMID #

N/A

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Summary

The Good

Easy to use like any elisa kit.

The Bad

Very long incubation periods, narrow concentration range, and quite expensive.

The Bottom Line

Reliable and reproducible way to detect ifn-g secretion, but takes a long time and optimization may be needed.

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