Reliable Antibody From BioLegend for Flow Cytometry Analysis

February 22, 2024

BlueskyReddit
Experimental Hematology
Department of Biomedicine, University Hospital Basel
Graduate Student

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Company:

BioLegend

Product Name:

APC anti-Human CD19

Catalog Number:

302212

Clone Number:

H1B19

Info:Supplier Page 

Browse Similar Products: Anti-CD19 Antibody Products

We aimed to label Raji lymphoma cells in a co-culture assay with T cells using flow cytometry. We employed Biolegend's APC anti-Human CD19 (H1B19) antibody to identify and quantify CD19-expressing Raji cells. This antibody consistently provided robust staining and clear discrimination of CD19-expressing cells, crucial for delineating B and T cell subsets within a coculture.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

PBMC derived primary human T cells

Primary Incubation

5µl antibody per million cells in 100 µl facs staining buffer for 30 minutes at 4 degrees.

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

Clear discrimination of CD19+ Raji cells with BioLegend's APC anti-Human CD19 (H1B19) antibody in a coculture with human CD3+ T cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Great price, bright signal.

The Bad

N/A

The Bottom Line

Recommended.

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