Satisfactory GLP-1 ELISA Kit From Millipore Sigma

UC Davis
Health Division of Gastroenterology

Overall

Quality of Results

Ease-of-Optimization

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Company:

Millipore

Product Name:

GLP-1 Total ELISA Kit

Catalog Number:

EZGLP1T-36K

We are interested in identifying orphan metabolites regulating mammalian physiology. We recently identified an exercise-inducible circulating lipid that has potential benefits in improving glucose metabolism. We therefore use this ELISA kit to determine if an injection of this metabolite in mice can affect GLP-1 abundance in blood plasma.

Experimental Design and Results Summary

Application

Quantifying mouse GLP-1

Starting Material

Blood plasma of mice

Tips

Aliquote enzymes and freeze down at -20 degrees C.

Results Summary

This ELISA kit is not the best kit for measuring mouse blood plasma GLP-1 because the plasma concentration is near the detection limit. 4 of out 10 fresh mouse plasma samples tested are below detection limit.

DOI or PMID #

N/A

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Summary

The Good

Easy to use.

The Bad

Not very sensitive.

The Bottom Line

This is a good ELISA kit for quantifying GLP-1 in cell culture but not very sensitive in detecting mouse plasma GLP-1.

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