Cytokine Array Analysis in Bovine LPS Activated Plasma

Oregon State University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

RayBiotech

Product Name:

Bovine Cytokine Array Q1

Catalog Number:

QAB-CYT-1

Clone Number:

I did cytokines analysis on cattle's plasma (LPS activated) using the Bovine Cytokine Array Q1. My research investigated the effect of feeding cattle with biomass from hemp-containing cannabinoids on inflammation, metabolism, etc. My professor asked me to try this product and it has an overall protocol that's very easy to follow. The overall time to run this protocol can be between 8-12 hours. I was able to get decent results using these assays.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Bovine plasma

Primary Incubation

2-3 hour

Blocking Agent

Quantibody® Sample Diluent

Secondary Incubation

2 hours

Tertiary Incubation

Biotinylated Antibody Cocktail

Detection

Fluorescence

Results Summary

Overall I was able to get the results from the fluorescence signal, although some background noise were there. But, the results were as expected.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Simple, and affordable.

The Bad

Not for absolute quantification (cannot expect absolute concentration).

The Bottom Line

Cheap and good for semi-quantitative purpose.

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