Myl9 Polyclonal Western Blot Antibody From Thermo Fisher Works Well

February 21, 2024

BlueskyReddit
KCL
Doctor of Philosophy

Overall

Quality of Results

Ease-of-Optimization

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Company:

ThermoFisher Scientific

Product Name:

Myl9 Polyclonal Antibody

Catalog Number:

PA5-17624

Clone Number:

This research involved looking at platelet markers using lysed whole blood via western blots in an acute infection model to see how they altered over the time course of infection.

Experimental Design and Results Summary

Applications

Western blot

Sample

Lysed whole blood

Primary Incubation

1:1000 dilution at 4 degrees overnight

Blocking Agent

Milk blocking buffer in TSB tween

Secondary Incubation

1:2000 dilution for 1 hour at room temperature

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

The results helped me show that myl9 protein expression levels altered over the time course of infection.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

This antibody was good once it could be detected.

The Bad

It took a lot of troubleshooting to be able to detect myl9 and the bands were not as strong.

The Bottom Line

This antibody works but is pricey to buy and hard to detect as its 20 kDa.

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