Decent Anti-Mouse T-bet eFluor™ 660 Antibody From eBioscience

February 27, 2024

Medicine
Weill Cornell Medicine
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

T-bet Monoclonal Antibody eFluor™ 660 eBioscience

Catalog Number:

50-5825-82

Clone Number:

eBio4B10 (4B10)

This antibody provides a good T-bet mouse staining on lymphocytes and an acceptable background. It allows good detection of mouse group 1 Innate lymphoid cells and NK cells.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse colonic cells

Primary Incubation

1/200 in PBS

Blocking Agent

Fc Block

Secondary Incubation

Intranuclear staining eBioscience FOXP3 Fix/Perm buffer.

Tertiary Incubation

None

Detection

BD LSR Fortessa II

Results Summary

I use this antibody for detecting group 1 innate lymphoid cells and NK cells among lymphocytes from mouse colon tissue. The staining was convincing and specific to perform proper gating strategy.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Decent and specific staining.

The Bad

The shift is just decent, you may need titration to get the best staining intensity.

The Bottom Line

Decent staining of T-bet-positive cells in lymphocytes isolated from mouse colon tissues. Great for detecting group 1 innate lymphoid cells and NK cells.

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