Decent Eomesodermin Monoclonal Antibody Alexa Fluor 488 From eBioscience

February 21, 2024

Medicine
Weill Cornell Medicine
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

EOMES Monoclonal Antibody Alexa Fluo 488

Catalog Number:

53-4875-82

Clone Number:

Dan11mag

This antibody provides a decent eomesodermin mouse staining on CD45-positive colonic live cells and an acceptable background. It allows good detection of mouse NK cells.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse colonic cells

Primary Incubation

1/200 in PBS

Blocking Agent

Fc Block

Secondary Incubation

Intranuclear staining eBioscience FOXP3 Fix/Perm buffer

Tertiary Incubation

None

Detection

BD LSR Fortessa II

Results Summary

I use this antibody for detecting NK and cytotoxic T cells among lymphocytes from mouse colonic tissues. The staining was decent and specific enough to perform proper gating strategy these cells subsets.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Decent and specific staining.

The Bad

The shift is just decent, you may need titration to get the best staining intensity.

The Bottom Line

Good staining of Eomesodermin-positive cells in CD45-positive cells isolated from mouse colonic tissues. Great for detecting NK cells and cytotoxic T cells.

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