Anti-Mouse CD64 Antibody From BioLegend Produces Good Results for the Characterization of Macrophages

February 07, 2024

BlueskyReddit
WCM
Postdoctoral Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 605™ anti-mouse CD64 (FcγRI) Antibody

Catalog Number:

139323

Clone Number:

This antibody gives good results for the characterization of macrophages in LPMCs by flow cytometry.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

LPMC

Primary Incubation

1:200 in FACS buffer, 20 min on ice

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

FACS

Results Summary

This antibody gives good results for the characterization of macrophages in LPMCs by flow cytometry at dilution 1:200 in FACS buffer. Signal could be a bit brighter but is in a convenient fluorochrome.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Reliable and bright staining.

The Bad

Could be a bit brighter.

The Bottom Line

Good antibody to use in standard phenotyping panel.

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