GFP Antibody From Invitrogen Works Well for Immunofluorescence

February 14, 2024

BlueskyReddit
Biochemistry
Stanford University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

GFP Polyclonal Antibody

Catalog Number:

PA5-141002

Clone Number:

I used this GFP antibody to label GFP-tagged NFAT5 protein in U2OS cells. The antibody shows good staining with minimum background.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

U2OS Cells

Primary Incubation

1:500 for 1 hr at 4 degrees C

Blocking Agent

BSA

Secondary Incubation

Gunieapig anti-rabbit AF594

Tertiary Incubation

None

Detection

Confocal imaging

Results Summary

I used this GFP antibody to label transiently transfected GFP-tagged NFAT5 protein in U2OS cells. The antibody shows good staining with minimum background.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specificity for the epitope.

The Bad

None.

The Bottom Line

Good antibody for weakly expressed GFP-Tagged proteins.

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