Sigma-Aldrich
Anti-RNA polymerase II Antibody
05-623
clone CTD4H8
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I study the transcriptional response in response to stress. I have used this antibody for labeling RNA polymerase II in cells.
Immunofluorescence
IMCD3 Cells
1:500 for 1 hr at 4 degrees C
BSA
Anti-mouse AF647
None
Confocal imaging
The cells show labeling of RNA polymerase II in the nucleus. The antibody has very little background. Also, since this antinody is generated from peptide containing 10 repeats of YSPT[pS]PS corresponding to the carboxyl-terminal domain of RNA polymerase II, the phosphorylated Serine helps to detect active pool of RNA polII during transcription.
N/A
Specificity for the epitope with minimum background.
None.
Good antibody to study transcriptional response.