Ideal Antibody From Biolegend for Lymphatic Endothelial Cell Staining

UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Anti-mouse Pe/Cy7 Podoplanin

Catalog Number:

127412

Clone Number:

8.1.1

This antibody stains CD45-negative cells well! I see good separation in my populations as podoplanin labels lymphatic endothelial cells.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse stromal cells

Primary Incubation

1:100, 4 degrees C for 30 minutes

Blocking Agent

Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

This product helped me identify lymphatic endothelial cells in the mouse lymph nodes by flow cytometry. It stains this population very well.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Great separation.

The Bad

None.

The Bottom Line

Definitely use this antibody for lymphatic endothelial cell staining!

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