Suitable Antibody to Stain Mediator Complex

February 13, 2024

BlueskyReddit
Biochemistry
Stanford University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Bethyl Laboratories

Product Name:

MED1 Recombinant Rabbit Monoclonal Antibody

Catalog Number:

A700-037

Clone Number:

BLR037F

I am studying eukaryotic transcription and used the Med1 antibody to label the mediator complex that takes part in transcription.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

IMCD3 Cells

Primary Incubation

1:500 for 1 hr at 4 degrees C

Blocking Agent

BSA

Secondary Incubation

Gunieapig anti-rabbit AF594

Tertiary Incubation

None

Detection

Confocal imaging

Results Summary

I used this antibody to label endogenous Med1 that is a part of the mediator complex involved in transcription. This antibody allowed me to look at transcriptional sites in cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Specificity for the epitope.

The Bad

None.

The Bottom Line

Good antibody to label endogenous Med1.

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