Mini Protein Gels From Invitrogen for Western Blot

February 05, 2024

BlueskyReddit
University of South Florida
College of Medicine
Postdoctoral Researcher

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Company:

Invitrogen™

Product Name:

Invitrogen™ Bolt™ Bis-Tris Plus Mini Protein Gels, 4‐12%, 1.0 mm, WedgeWell™ format

Catalog Number:

NW04122BOX

We study the CNS basement membrane and blood-brain barrier. Specifically the role of different CNS cell types in BBB maintenance.

Experimental Design and Results Summary

Application

Electrophoresis & Western Blotting

Starting Material

Mouse Tissue protein

Tips

Bolt Bis-Tris Plus gels can be run using constant voltage in approximately 20 minutes with the Bolt MES running buffer. Bolt Bis-Tris Plus gels offer sharper bands, high resolution, longer shelf life, and higher accuracy of results than Tris-glycine–based gels. In addition, Bolt Bis-Tris Plus gels have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0-mm thick gels. The wedge-shaped wells also have larger openings, making sample loading easier. And Bolt Bis-Tris Plus gels have a shelf life of at least 16 months at 4°C to 25°C.

Results Summary

We use this Invitrogen Bolt Bis-Tris Plus gels for optimal separation of a wide range of protein sizes under denaturing conditions.

DOI or PMID #

N/A

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Summary

The Good

Use Bolt Bis-Tris Plus gels to obtain optimal results for your day-to-day protein separation needs. Bolt Bis-Tris Plus gels come in four gel types and multiple well formats.

The Bad

Higher cost.

The Bottom Line

Bolt Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications with increased loading capacity. Bolt gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial.

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