KAPA LongRange Kit From Roche Produces High Quality Results

January 29, 2024

BlueskyReddit
University of South Florida
College of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Roche

Product Name:

KAPA LongRange Hotstart PCR Kit

Catalog Number:

KK3503

We study the CNS basement membrane and blood-brain barrier. Specifically the role of different CNS cell types in BBB maintenance.

Experimental Design and Results Summary

Application

PCR and QPCR

Starting Material

Tail DNA, Purified DNA

Tips

KAPA Long Range is also supplied in 2X ReadyMix format with loading dye that contains all the components required for PCR except primers and template.

Results Summary

We use this KAPA reagent for amplification of targets with high sensitivity and specificity up to 15 kb. It provides high yields with low-input DNA.

DOI or PMID #

N/A

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Summary

The Good

High Quality Results.

The Bad

Nothing.

The Bottom Line

KAPA LongRange has been used in the amplification of long targets and/or low amounts, Standard short- and mid-range PCR, T-A cloning, Targeted sample enrichment for next-generation DNA sequencing polymerase chain reaction (PCR).

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