Decent Anti-Mouse Ly6G Antibody From eBioscience

January 04, 2024

BlueskyReddit
Medicine
Weill Cornell Medicine
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-mouse Ly-6G Monoclonal Antibody Alexa Fluor™ 700

Catalog Number:

56-5931-82

Clone Number:

RB6-8C5

This antibody provides a good Ly6G mouse staining on CD45-positive cells and an acceptable background. It allows good detection of mouse Granulocytes

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse caecum cells

Primary Incubation

1/200 in PBS

Blocking Agent

Fc Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR Fortessa II

Results Summary

I use this antibody for detecting LY6G-positive granulocytes cells among tumor-infiltrating immune cells from mouse with spontaneous APCf/f caecal tumors. The staining was convincing and specific to perform proper gating strategy.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Decent and specific staining.

The Bad

Require antibody titration to improve the staining at the best.

The Bottom Line

Good staining of Ly6G in mouse tumor-infiltrating immune cells in a mouse model of caecal tumors. Great for detecting Ly6G-positive granulocytes.

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