Cell Signaling Technology
E-Cadherin (24E10) Rabbit mAb (Alexa Fluor® 594 Conjugate)
#7687
24E10
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Good quality antibody with minimal background fluorescent, and long-lasting signal post-mounting. It gave good structural clarity and was specific in staining.
Immunofluorescence
Cancer Cell lines
Overnight (16 hours)
5% goat serum, 3% BSA
N/A
Confocal microscopy
Good structural staining for E-cadherin in cancer cell lines, minimal non-specific binding. Fluorescent signals were long-lasting post-mounting.
Minimal non-specific staining.
Quite expensive but it is conjugated so no secondary needed.
It requires a 1:50 dilution but offers long-lasting fluorescent compared to other conjugated primaries.