Easiest ROCK Inhibitor From Gibco for Thawing and Passing IPSCs

City University of New York

Overall

Quality of Results

Ease-of-Optimization

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Company:

ThermoFisher

Product Name:

RevitaCell supplement

Catalog Number:

A2644501

I use this post-thaw or if I pass with accutase to reduce cell death. I prefer it to other ROCK inhibitors because it comes premade and is very easy to use and since iPSC cultures are already so demanding simplifying one step is nice. Like any ROCK inhibitor, remove within 24 hrs to avoid spontaneous differentiation.

Experimental Design and Results Summary

Application

Cell culture

Starting Material

N/A

Tips

Remove within 24 hrs

Results Summary

Increases cell survival but does give your cells a slightly "spikier" morphology that goes away once you change the media to remove the revitacell.

DOI or PMID #

N/A

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Summary

The Good

Very easy to use.

The Bad

More expensive than making your own ROCK inhibitor solution.

The Bottom Line

Simplest way to increase iPSC survival post thaw.

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