Convenient Reagent From Thermo Fisher for Delayed RNA Extraction

George Washington University
Cancer Center
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermofisher

Product Name:

RNAlater

Catalog Number:

AM7020

The goal of this experiment was to evaluate changes in dendritic cell transcriptome after exposure to lysed tumor cells.

Experimental Design and Results Summary

Application

RNA isolation/sequencing

Starting Material

Cells

Tips

Add RNAlater in 5:1 RNAlater to cell suspension ratio

Results Summary

This product helped us isolate RNA at a later date, which allowed for sequencing of RNA from multiple experiments within the same run, reducing batch effect.

DOI or PMID #

N/A

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Summary

The Good

Easy to use.

The Bad

Slightly less recovery than isolating RNA directly.

The Bottom Line

Great product for making your day-to-day easier by allowing RNA isolation at a later date.

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