Astrocyte Isolation MicroBead Kit From Miltenyi Biotec

December 26, 2023

BlueskyReddit
State University of New York at Buffalo
Physiology and Biophysics
Postdoctoral Researcher

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Company:

Miltenyi Biotec

Product Name:

Anti-ACSA-2 MicroBead Kit, mouse

Catalog Number:

130-097-678

We use the Anti-ACSA-2 MicroBead Kit to isolate brain astrocytes from adult mice for downstream analyses, including western blot and metabolic flux assays.

Experimental Design and Results Summary

Application

Cell Isolation

Starting Material

Mouse brain

Tips

For optimal performance, it is important to obtain a single-cell suspension before magnetic labeling.

Results Summary

Astrocytes were acutely isolated from the whole brain using magnetic bead technology, optimized to yield pure astrocytes as validated by the expression of the astrocyte-specific marker (S-100), but not neuronal (NeuN) or microglial (Iba-1) markers.

DOI or PMID #

37408246; 35275429

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Summary

The Good

High purity of isolated astrocytes.

The Bad

Expensive.

The Bottom Line

Anti-ACSA-2 MicroBead Kit from Miltenyi Biotec is a great kit for isolation of high purity astrocytes from mouse adult brain. We use it routinely in the lab for numerous downstream applications, including cultivation of astrocytes, Seahorse assay, RNA seq and western blotting.

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