Highly Recommended Anti-Mouse CD3 AF488 Antibody From Invitrogen

Molecular and Cell Biology
UC Berkeley
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

Anti-mouse CD3 AF488 antibody

Catalog Number:

53-0032-80

Clone Number:

I used this CD3 antibody to stain T cells in the lymph nodes for IF. It works better than FITC color for imaging and worked well for our purpose of distinguishing T and B cell zones in the tissue.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse lymph nodes

Primary Incubation

1:500, 1hr, RT

Blocking Agent

Blockaid

Secondary Incubation

None

Tertiary Incubation

None

Detection

Immunofluorescence

Results Summary

I used this CD3 antibody to stain for T cells in the lymph nodes for IF. It works better than FITC color for imaging and worked well for our purpose to distinguish T and B cell zones in the tissue.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Reliable and good signal.

The Bad

None.

The Bottom Line

Antibody has great signal, highly recommend.

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