Decent Anti-Mouse CD3 FITC Antibody From Tonbo Bioscience

Molecular and Cell Biology
UC Berkeley
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Tonbo Bioscience

Product Name:

Anti-mouse CD3 FITC antibody

Catalog Number:

35-0032-U100

Clone Number:

I used this antibody to stain T cells in the mouse lymph nodes for immunofluorescence. It was a little dim but still looked okay if you turned the channel up more. It looks exclusive from the B cell marker on the image so it worked well.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse lymph nodes

Primary Incubation

1:500, 1hr, RT

Blocking Agent

Blockaid

Secondary Incubation

None

Tertiary Incubation

None

Detection

Immunofluorescence

Results Summary

I used this antibody to stain for T cells in the mouse lymph nodes for immunofluorescence. It was a little dim but still looked okay if you turn the channel up more. It looks exclusive from B cell marker on the image so it worked well.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Reliable and good signal.

The Bad

None.

The Bottom Line

Antibody works well, would recommend.

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