Great Antibody From BD Biosciences With Good Staining

Surgery
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BD Biosciences

Product Name:

Anti-mouse CD3e BUV395 antibody

Catalog Number:

563565

Clone Number:

We always use this antibody to stain for T cells in the mouse lymphoid organs. It can be dim sometimes in the tissues so we usually use it to dump out T cells to enrich for more myeloid cells. If you want to analyze T cells, this antibody might be too dim.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymph nodes

Primary Incubation

1:200, 30min, 4C

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

We always use this antibody to stain for T cells in the mouse lymphoid organs. It can be dim sometimes in the tissues so we usually use it to dump out T cells to enrich for more myeloid cells. If you want to actually analyze T cells, this antibody might be too dim.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Reliable and good signal.

The Bad

Signal somewhat dim.

The Bottom Line

This antibody works well, would recommend.

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