Good Antibody for B Cells Detection From Miltenyi Biotec

PRIMATE RESEARCH CENTER
UNIVERSITY OF WISCONSIN
Scientist I

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

Human CD19 antibody

Catalog Number:

130-113-170

Clone Number:

LT19

The CD19 antibody from Miltenyi is a very good antibody for the detection of B cells from the in-vitro differentiation of iPSC/ESCs.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Human ESC/iPSC derived B cells

Primary Incubation

1ul in 100ul of FACS buffer with 1e6 cells

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

This clone of CD19 antibody works very well to detect CD19 positive B cells generated from lymphoid differentiation of hematopoietic progenitors. The antibody clearly separates positive from negative cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very easy to use.

The Bad

None

The Bottom Line

The LT-19 clone of CD19 antibody can very a very good antibody to detect CD19 positive cells in Bcell lymphoid differentiation.

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