Abcam
Anti-Ki67 antibody
ab16667
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The specific goal of this experiment was to evaluate the effects of the drug on mouse fibroblast proliferation. We wanted to use a Ki-67 rabbit antibody. This rabbit antibody provided fair staining for the Ki-67 protein that is expressed in proliferative cells.
Immunofluorescence
Mouse fibroblast
1:200 at 4 degrees Celsius for over night
10% normal donkey serum
Secondary antibody at 1:500 in 10% serum for 1 hour at room temperature
N/A
Fluorescence
Immunofluorescence labeling with this antibody generated fluorescence signals to Ki67 expressed in proliferative fibroblasts (green). There was non specific background observed with this antibody.
Reliable signals.
Higher background.
This antibody may be a good option for researchers looking for a rabbit Ki67 antibody that works for immunofluorescence.